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Comparison of the growth of Escherichia coli cells transformed with empty <t>pET21a(+)</t> vector and pET21a(+) vectors harbouring coding regions of AsMT1-4 in LB medium (control) and LB medium supplemented with Zn or Cd ions. The expression of AsMT1-4 was induced by 0.1 mM IPTG. The relative growth rate is expressed as a slope of bacterial growth curves obtained by plotting optical density against time. Bars represent means ( n = 9) ± SE. The results obtained for a given condition were compared, and distinct letters indicate significant differences between E. coli carrying different plasmids (Kruskal–Wallis, Mann–Whitney; p < 0.05).
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pet21a expression vector  (Millipore)
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Comparison of the growth of Escherichia coli cells transformed with empty <t>pET21a(+)</t> vector and pET21a(+) vectors harbouring coding regions of AsMT1-4 in LB medium (control) and LB medium supplemented with Zn or Cd ions. The expression of AsMT1-4 was induced by 0.1 mM IPTG. The relative growth rate is expressed as a slope of bacterial growth curves obtained by plotting optical density against time. Bars represent means ( n = 9) ± SE. The results obtained for a given condition were compared, and distinct letters indicate significant differences between E. coli carrying different plasmids (Kruskal–Wallis, Mann–Whitney; p < 0.05).
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Comparison of the growth of Escherichia coli cells transformed with empty pET21a(+) vector and pET21a(+) vectors harbouring coding regions of AsMT1-4 in LB medium (control) and LB medium supplemented with Zn or Cd ions. The expression of AsMT1-4 was induced by 0.1 mM IPTG. The relative growth rate is expressed as a slope of bacterial growth curves obtained by plotting optical density against time. Bars represent means ( n = 9) ± SE. The results obtained for a given condition were compared, and distinct letters indicate significant differences between E. coli carrying different plasmids (Kruskal–Wallis, Mann–Whitney; p < 0.05).

Journal: Foods

Article Title: The Contribution of Trichoderma viride and Metallothioneins in Enhancing the Seed Quality of Avena sativa L. in Cd-Contaminated Soil

doi: 10.3390/foods13152469

Figure Lengend Snippet: Comparison of the growth of Escherichia coli cells transformed with empty pET21a(+) vector and pET21a(+) vectors harbouring coding regions of AsMT1-4 in LB medium (control) and LB medium supplemented with Zn or Cd ions. The expression of AsMT1-4 was induced by 0.1 mM IPTG. The relative growth rate is expressed as a slope of bacterial growth curves obtained by plotting optical density against time. Bars represent means ( n = 9) ± SE. The results obtained for a given condition were compared, and distinct letters indicate significant differences between E. coli carrying different plasmids (Kruskal–Wallis, Mann–Whitney; p < 0.05).

Article Snippet: The PCR products were digested with Nde I and Xho I, and ligated into a pET21a(+) expression vector (Novagen, Darmstadt, Germany) to be later transformed into E. coli DH5α.

Techniques: Comparison, Transformation Assay, Plasmid Preparation, Control, Expressing, MANN-WHITNEY